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The kit adopts interferon-γ release assay for Mycobacterium tuberculosis (TB-IGRA) to measure the intensity of the cellular immune response mediated by Mycobacterium tuberculosis specific antigen. The Enzyme-linked immunosorbent assay and double antibody sandwich principle. • The microplates are pre-coated with anti IFN-γ antibodies. • The specimens to be tested are added into the antibody coated microplate wells, then horseradish peroxidase (HRP)-conjugated anti IFN-γ antibodies are added into the respective wells. • IFN-γ, if present, will form a sandwich complex with anti IFN-γ antibodies and HRP-conjugated anti IFN-γ antibodies. • Color will be developed after adding the substrate solutions, and will change after adding the stop solutions. The absorbance (OD) is measured with an ELISA reader. • The IFN-γ concentration in the sample is correlated to the determined OD.Effective diagnostic ELISA for latent and active TB infection No interference from BCG vaccine
