Get accurate results with our EV71 IgG ELISA Kit! As a factory, we ensure high-quality tests for enterovirus detection. Order now and trust the experts!
This kit detects Enterovirus 71 IgG antibody (EV71-IgG) in human serum or plasma samples, polystyrene microwell strips are pre-coated with Enterovirus 71 antigen. After first adding serum or plasma specimens to be examined, corresponding specific antibodies (EV71-IgG-Ab & some IgM-Ab) present in patient specimens bind to the antigens at the solid phase, and other unbound components will be removed by washing. In the second step, the HRP (horseradish peroxidase)-conjugated anti-human IgG will specifically react only with EV71 IgG antibodies. After washing to remove the unbound HRP-conjugate, chromogen solutions are added into the wells. In presence of (EV71 Ag) - (EV71-IgG) - (anti-human IgG-HRP) immunocomplex, after washing the plate, the TMB substrate was added for color development, and the HRP connected to the complex catalyzes the color developer reaction to generate the blue substance, add 50μL of Stop Solution, and turn yellow. The presence of the absorbance of the EV71-IgG antibody in the sample was determined by a microplate reader.High sensitivity, specificity and stability
